Aryl Hydrocarbon Receptor (AhR) Assay Kit
CELL-BASED REPORTER ASSAY
Aryl Hydrocarbon Receptor (AhR) Assay Kit
Investigate human AhR signaling using a luciferase reporter assay designed to evaluate receptor activation and inhibition in cell-based research.
Aryl Hydrocarbon Receptor (AhR) Assay Kit
SKU: IB06001
Product Information
Product: Human Aryl Hydrocarbon Receptor (AhR) Assay Kit
Main SKU: IB06001
Related SKUs: IB06001-32, IB06002
Storage: -80°C, subject to supplier specifications.
Available Assay Formats
| SKU | Format |
|---|---|
| IB06001-32 | 3 × 32 assays, 96-well format |
| IB06001 | 96-well format |
| IB06002 | 384-well format |
Product Description
The Aryl Hydrocarbon Receptor (AhR) Assay Kit is a cell-based reporter system for investigating the activity of the human AhR signaling pathway. It uses reporter cells containing a luciferase gene controlled by an AhR-responsive promoter.
When a compound activates the pathway, luciferase expression generates a measurable luminescent signal. Changes in this signal can help researchers evaluate receptor activation or inhibition under appropriate experimental conditions.
Key Features
- Luciferase-based luminescence detection.
- Human AhR agonist screening.
- AhR antagonist evaluation.
- 96-well and 384-well assay formats, depending on SKU.
- Functional investigation of chemical and environmental compounds.
Research Applications
- AhR pathway activation studies.
- Compound screening and dose-response analysis.
- Investigation of xenobiotic-responsive signaling.
- Research into transcriptional regulation.
- Cell-based functional assays.
How the AhR Reporter Assay Works
1. Reporter cells are exposed to test compounds.
2. Active compounds may stimulate AhR-dependent signaling.
3. The reporter gene produces luciferase.
4. A suitable luminometer measures the resulting light signal.
5. Results are compared with appropriate experimental controls.
Technical Specifications
Assay principle: Cell-based luciferase reporter assay
Target: Human aryl hydrocarbon receptor (AhR)
Detection method: Luminescence
Functional endpoints: AhR agonist and antagonist activity
Plate formats: 96-well and 384-well, depending on SKU
Explore Human AhR Functional Assays
Choose the assay format that suits your research workflow. Consult the supplier's technical documentation for complete specifications and handling instructions.
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