News

Sandwich ELISA :  Principle, Steps, Applications, Advantages and Limitations Introduction to Sandwich ELISA Sandwich ELISA (Enzyme-Linked Immunosorbent Assay) is a highly specific and sensitive immunoassay technique used to detect and quantify antigens in biological samples. It is called “sandwich” ELISA because the target antigen is captured between two layers of antibodies. This method is widely used in diagnostics, research, and biotechnology, especially for detecting protei …

Radioimmunoassay (RIA)  Principle, Procedure, Applications, Advantages and Limitations Introduction to Radioimmunoassay (RIA) Radioimmunoassay (RIA) is a highly sensitive laboratory technique used to measure minute concentrations of antigens (such as hormones, drugs, and proteins) in biological samples. It combines the specificity of antigen-antibody interactions with the sensitivity of radioactive detection. Developed in the 1960s by Rosalyn Yalow and Solomon Berson, RIA revolutionized cli …

Reverse Transcriptase PCR: A Complete Guide for Researchers Introduction to Reverse Transcriptase PCR Reverse transcriptase PCR (RT-PCR) is a powerful molecular biology technique used to detect and quantify RNA. Unlike traditional PCR, which amplifies DNA, RT-PCR starts with RNA as a template and converts it into complementary DNA (cDNA) using the enzyme reverse transcriptase. This method allows scientists to study gene expression, detect viral infections, and analyze RNA-based biomarkers with h …

To generate cDNA (complementary DNA) Gentaur uses these steps:  Isolation of RNA: Extract RNA from the desired tissue or cell samples. This can be done using commercially available RNA isolation kits, such as TRIzol or RNeasy kits. Ensure that the RNA isolation method chosen is appropriate for the specific sample type.Reverse Transcription (RT): Perform reverse transcription to convert the isolated RNA into cDNA. This involves using reverse transcriptase enzyme to synthesize cDNA …