Digital PCR (dPCR)

In recent years, digital PCR (dPCR) has undergone significant advancements that have revolutionized its capabilities and broadened its applications.

Schemes of typical digital PCR (dPCR) workflow


One key area of progress is the development of high-throughput dPCR platforms, which allow for the simultaneous analysis of thousands of samples. This high-throughput capability is particularly valuable in fields such as infectious disease surveillance, where the rapid and accurate detection of pathogens is crucial.

Comparison of commercial dPCR platforms

Another major advancement is the improvement in multiplexing capabilities, which enable the detection of multiple targets in a single reaction. This is especially useful in oncology research, where the detection of multiple genetic mutations or biomarkers in a single sample can provide valuable insights into tumor heterogeneity and treatment response.

Single-cell dPCR techniques have also seen significant advancements, allowing researchers to analyze genetic and epigenetic variations at the single-cell level. This level of sensitivity and precision is invaluable in fields such as developmental biology and cancer research, where understanding cellular heterogeneity is critical.

Direct Assessment of Single-Cell DNA Using Crudely Purified Live Cells

Furthermore, the integration of dPCR with digital spatial profiling technologies has opened up new possibilities for spatially resolved molecular profiling. This technology allows researchers to map the distribution of biomolecules within tissue samples, providing insights into complex biological processes such as tumor microenvironments and immune responses.

Overall, these advancements have positioned dPCR as a powerful and versatile tool with broad applications in basic research, clinical diagnostics, and personalized medicine. As technology continues to evolve, dPCR is expected to play an increasingly important role in advancing our understanding of complex biological systems and improving patient care.


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