Small RNA Library Prep Kit for Illumina

Small RNA Sequencing & Library Preparation

Small RNA Library Prep Kit for Illumina (Indexes 25–48)

Small RNA Library Preparation Kit for Next-Generation Sequencing of Low-Input RNA Samples

Product Overview

The Small RNA Library Prep Kit for Illumina (Indexes 25–48) is designed to prepare small RNA libraries for next-generation sequencing (NGS) using Illumina sequencing platforms. The kit is optimized for low-input RNA samples, including RNA obtained from plasma, serum, urine, and other liquid biopsy specimens.

It provides the essential molecular reagents required for library construction, including adapters, primers, enzyme mixes, buffers, and a purification module. Its streamlined workflow supports rapid library preparation while helping recover nucleic acid intermediates and final library products.

Key Highlight: Optimized for low-input RNA, with library preparation possible in less than 5 hours.

Key Features & Benefits

Low RNA Input

Optimized for low RNA quantities, including samples containing approximately 1 ng of RNA.

Fast Workflow

A streamlined library preparation workflow that can be completed in less than 5 hours.

Integrated Purification

Includes a purification module for recovering library intermediates and final products.

Complete Reagent Set

Includes adapters, primers, enzyme mixes, buffers, and other required components.

Compatible RNA Sample Types

The workflow is designed to accommodate different RNA inputs, including purified total RNA, enriched small RNA, and low-content biological samples.

Plasma RNA

Serum RNA

Urine RNA

Liquid Biopsy RNA

Purified Total RNA

Enriched Small RNA

Research Applications

MicroRNA (miRNA) Sequencing: Preparation of small RNA libraries for microRNA profiling.
Small RNA Expression Analysis: Investigation of small RNA expression patterns and abundance.
Liquid Biopsy Research: Small RNA analysis using plasma, serum, and urine samples.
Biomarker Discovery: Identification and characterization of potential RNA biomarkers.
Next-Generation Sequencing: Preparation of sequencing-ready libraries for Illumina platforms.
Non-Coding RNA Research: Investigation of small regulatory RNA molecules and their biological functions.

Small RNA Library Preparation Workflow

RNA Sample Input

Adapter Ligation

cDNA Synthesis & Amplification

Library Purification

Illumina Sequencing

Illustrative overview of a typical small RNA library preparation process. Follow the supplied kit protocol for the exact procedure.

Technical Specifications

Product Name	Small RNA Library Prep Kit for Illumina (Indexes 25–48)
Catalog Number	1111-63620
Pack Size	24 Preparations
Index Range	25–48
Minimum Order Quantity	1
UNSPSC	41105905
UN Code	UN1845
Dry Ice	Yes
Hazardous	Yes
Shipping Conditions	Dry Ice
Storage Conditions	Freezer
Storage Temperature	-20 °C
Shelf Life	At least 1 year when stored under specified conditions
Weight	28

Storage & Handling

Store the kit in a freezer at -20 °C under the specified storage conditions. The reagents should remain stable for at least one year when properly stored. The product is shipped on dry ice. Follow the manufacturer's instructions for handling, storage, and laboratory safety.

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