FNC Coating Mix®, 100 ml

Cell Culture & Surface Coating

FNC Coating Mix®, 100 ml

Serum-Free Cell Attachment Reagent for Enhanced Adherent Cell Culture

FNC Coating Mix 100 ml cell culture coating reagent

Product Overview

FNC Coating Mix® is a specially formulated serum-free tissue culture reagent designed to enhance the attachment of adherent mammalian cells to plastic culture surfaces, including flasks, dishes and microplates.

Its formulation contains fibronectin, collagen and albumin, creating an extracellular matrix-like coating that supports cell attachment and monolayer formation. It is particularly useful for cell types that are difficult to attach and propagate under standard culture conditions, including certain human epithelial cell models.

When used with compatible serum-free culture media, FNC Coating Mix® helps provide a suitable surface environment for adherent cell growth and cell-based research.

Key Highlight: A serum-free coating formulation designed to improve adherent cell attachment and support efficient monolayer formation.

Key Features & Benefits

Enhanced Cell Attachment

Improves the attachment of adherent mammalian cells to plastic culture surfaces.

Serum-Free Formulation

Designed for cell culture workflows using compatible serum-free culture media.

Extracellular Matrix Components

Contains fibronectin, collagen and albumin to support a cell-compatible coated surface.

Ready-to-Use Liquid

Convenient coating reagent for preparing cell culture surfaces.

Monolayer Formation

Supports cell attachment and the development of adherent cell monolayers.

Fastidious Cell Types

Useful for certain epithelial and other adherent cells requiring enhanced substrate attachment.

Composition & Coating Properties

FNC Coating Mix® combines three important components associated with extracellular matrix and cell attachment:

Fibronectin

An extracellular matrix glycoprotein involved in cell adhesion and interactions with the culture substrate.

Collagen

A structural extracellular matrix protein that contributes to cell-supportive surface properties.

Albumin

A protein component included in the formulation to support its coating properties.

Research Applications

  • Adherent Cell Culture: Preparation of coated plastic surfaces to improve attachment of adherent mammalian cells.
  • Epithelial Cell Culture: Supports attachment and growth of epithelial cell models, including difficult-to-attach cell types.
  • Primary Cell Culture: Used as a substrate coating in suitable primary adherent cell culture workflows.
  • Cell Monolayer Formation: Helps support efficient attachment and establishment of adherent cell monolayers.
  • Cell Biology Research: Suitable for experimental tissue culture and cell-based research workflows.
  • Specialized Cell Models: Has been used in research involving human corneal endothelial cells, renal epithelial cells and other adherent cell models.

Typical Coating Workflow

FNC Coating Mix® can be used to prepare plastic culture surfaces before introducing adherent cells.

01. Coat

Cover the culture surface completely with FNC Coating Mix®.

02. Incubate

Allow the coating solution to contact the surface according to the applicable protocol.

03. Remove Excess

Aspirate the remaining coating solution.

04. Seed Cells

Add the prepared cell suspension to the coated culture surface.

Coating Reference: The associated manufacturer information describes an approximate coating volume of 0.2 mL/cm² and an incubation time of about 30 seconds before removing the excess solution. Always consult the applicable product protocol for detailed instructions.

Technical Specifications

Product Name FNC Coating Mix®
Catalog Number 0016-0407
Pack Size 100 mL
Formulation Serum-free liquid coating reagent
Main Components Fibronectin, collagen and albumin
Minimum Order Quantity 1
HS Code 3821000000
Dry Ice No
Hazardous No
Shipping Conditions Cryopacks
Storage Conditions 4 °C
Shelf Life 2 Years

Frequently Asked Questions

1. What is FNC Coating Mix®?

FNC Coating Mix® is a serum-free cell attachment reagent used to coat plastic culture surfaces and enhance the attachment of adherent mammalian cells.

2. What does FNC Coating Mix® contain?

It contains fibronectin, collagen and albumin, which contribute to its extracellular matrix-like coating properties.

3. What is FNC Coating Mix® used for?

It is used to improve cell attachment and support spreading and monolayer formation in suitable adherent cell culture systems.

4. Can FNC Coating Mix® be used for epithelial cells?

Yes. It is particularly useful for certain epithelial cell cultures and other adherent cells that require improved attachment to culture surfaces.

5. Is FNC Coating Mix® ready to use?

Yes. It is supplied as a liquid coating reagent for convenient preparation of culture surfaces.

6. What culture surfaces can be coated with FNC Coating Mix®?

It is designed for plastic cell culture surfaces, including flasks, dishes and microplates.

Related Products

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Order FNC Coating Mix®, 100 ml

Support efficient adherent cell culture with a specialized serum-free cell attachment coating reagent.

24/48H Stock Items & 2 to 6 Weeks Non-Stock Items

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10th Nov 2026

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