Product Information

Product name: Desmoglein-3 (Squamous Cell Marker) (DSG3/2837), CF405S Conjugate

Catalog number: 0037-BNC042837-500

Size: 1 × 500 µL

Concentration: 0.1 mg/mL

Host: Mouse

Species reactivity: Human

Clonality: Monoclonal

Clone: DSG3/2837

Antibody type: Primary antibody

Conjugate: CF405S fluorescent dye

Verified application: Immunohistochemistry, FFPE

Dry ice: No

Hazardous shipping: No

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

What Is Desmoglein-3 (DSG3)?

Desmoglein-3 (DSG3) is a calcium-binding transmembrane glycoprotein belonging to the cadherin family. It is a component of desmosomes, specialized cell junctions that help maintain adhesion between neighboring epithelial cells. These structures contribute to tissue integrity and the organization of epithelial layers.

The DSG3 protein is involved in cell adhesion and epithelial tissue biology. Its expression is also relevant to cancer pathology research, particularly studies investigating squamous differentiation and squamous cell carcinoma.

DSG3 as a Squamous Cell Carcinoma Marker

Desmoglein-3 is used as an immunohistochemical marker to investigate squamous differentiation in tissue samples. DSG3 staining can help researchers characterize squamous cell carcinoma and support the distinction between squamous tumors and other tumor types when interpreted alongside tissue morphology and additional markers.

In lung cancer research, DSG3 expression has been investigated as a marker associated with lung squamous cell carcinoma. Its interpretation depends on the tissue context, staining protocol, and other pathological findings. It should not be used as the sole basis for a diagnosis or prognosis.

Key Product Features

• Target specificity: Recognizes Desmoglein-3, a protein with a reported molecular weight of approximately 130 kDa.
• Monoclonal antibody: Mouse monoclonal antibody, clone DSG3/2837.
• Fluorescent labeling: CF405S conjugate for blue fluorescence-based detection.
• Human target reactivity: Intended to recognize human Desmoglein-3.
• Verified application: Immunohistochemistry on formalin-fixed, paraffin-embedded tissue.
• Defined concentration: Supplied at 0.1 mg/mL in a 500 µL format.
• Research utility: Suitable for research investigating epithelial biology and squamous cell carcinoma markers.

Research Applications

• Immunohistochemistry of formalin-fixed, paraffin-embedded tissue samples.
• Research on lung squamous cell carcinoma and other squamous cell carcinomas.
• Investigation of epithelial cell adhesion and desmosomal proteins.
• Studies of tumor marker expression and tissue differentiation.
• Histological research using appropriate controls and complementary markers.

Understanding the CF405S Fluorescent Conjugate

CF405S is a blue fluorescent dye used to visualize antibody labeling. Fluorescent conjugates can help researchers detect target proteins in appropriately prepared samples, although signal intensity depends on target abundance, sample preparation, background fluorescence, and imaging settings.

The supplied product information notes that blue fluorescent conjugates such as CF405S may be less suitable for detecting low-abundance targets because they can produce lower fluorescence and potentially greater nonspecific background than some other dye colors. Protect the conjugate from light and follow the manufacturer's recommended staining and imaging protocol.

Technical Specifications

Handling and Safety

• Protect the fluorescent conjugate from light during handling and storage.
• The buffer contains 0.05% sodium azide. Avoid ingestion and contact with skin or eyes. Follow the product safety data sheet and laboratory safety procedures.
• Use suitable positive and negative controls to assess staining specificity.
• The supplied listing mentions short-term stability at room temperature or 37°C for seven days. This should not be interpreted as the recommended long-term storage condition; consult the manufacturer's current instructions.

Frequently Asked Questions

What does the Desmoglein-3 antibody detect?

It recognizes Desmoglein-3, a desmosomal protein involved in adhesion between epithelial cells.

What is the main application of this antibody?

The listed verified application is immunohistochemistry on formalin-fixed, paraffin-embedded tissue samples.

Why is DSG3 studied in squamous cell carcinoma?

DSG3 expression can support the investigation of squamous differentiation and help characterize squamous cell carcinoma when used alongside histology and other markers.

What is the purpose of the CF405S conjugate?

CF405S provides blue fluorescence for visualization of antibody labeling. Its suitability depends on target abundance, sample background, and the fluorescence detection setup.