Rat Mammary Gland, non pregnant Total Protein | RT-414

Rat Mammary Gland, non pregnant Total Protein | RT-414 | Zyagen

Protein lysate is isolated from freshly harvested mammary gland. Tissues are homogenized in protein lysis buffer supplemented with a cocktail of 7 mammalian protease inhibitors to minimize proteolysis, precisely quantified, and stored at -80oC.

Applications: Total protein is ready for immediate use in Western blotting, immunoprecipitation, SDS-PAGE, isoelectric focusing gels and SDS-capillary electrophoresis, enzymatic activity analysis, gel shifting assay, protein-protein interaction, and tissue specific expression.

Quality Control: Quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of the protein bands is verified by denatured SDS-PAGE using Coomassie blue staining. The integrity of protein is tested by immunoblotting using specific beta-actin antibody.

Packing/Shipping/Storage: The extracted protein is provided in vials at a concentration of 5mg/ml and shipped on dry ice. Protein can also be provided in denatured SDS Sample buffer for only Western blotting use