Rat Mammary Gland Frozen Sections, E12 | RF-414-12

Rat Mammary Gland Frozen Sections, E12 | RF-414-12 | Zyagen

Tissues are freshly snapped frozen in OCT blocks, sectioned at a thickness of 7-10microns and sections are mounted on positively charged slides (2 sections per slide). For fixed frozen sections, tissues are fixed in 10% neutral buffered formalin or 4% paraformaldehyde, cryoprotected in sucrose solution, snapped frozen in OCT and sectioned as above. Frozen sections are shipped in tightly closed slide boxes or slide mailers on dry ice and are good for one year if properly stored.

Applications: Frozen tissue sections are prepared in RNase-, protease-free environment and are ideal for immunohistochemistry and in situ hybridization.

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