Rat Bone, Calvaria Total RNA | RR-108

Rat Bone, Calvaria Total RNA | RR-108 | Zyagen

Total RNA is routinely extracted from single healthy normal donor using the powerful classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop spectrophotometer (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction.. Total RNA sample is routinely provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.

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