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Introduction to Reverse Transcriptase PCR Reverse transcriptase PCR (RT-PCR) is a powerful molecular biology technique used to detect and quantify RNA. Unlike traditional PCR, which amplifies DNA, RT-PCR starts with RNA as a template and converts it into complementary DNA (cDNA) using the enzyme reverse transcriptase. This method allows scientists to study gene expression, detect viral infections, and analyze RNA-based biomarkers with high sensitivity. How Reverse Transcriptase PCR Works The p …