FITC Anti-Mouse CD16/32 Antibody

FITC Anti-Mouse CD16/32 Antibody · 25 µg

Product Overview

FITC Anti-Mouse CD16/32 Antibody is a fluorescently labeled monoclonal antibody from Reddot Biotech designed to recognize mouse CD16 and CD32, two Fc gamma receptors involved in immune regulation. Conjugated with fluorescein isothiocyanate (FITC), this antibody enables fluorescent detection of receptor-expressing cells by flow cytometry.

CD16, also known as Fc gamma receptor III (FcγRIII), and CD32, also known as Fc gamma receptor II (FcγRII), participate in interactions with the Fc regions of immunoglobulin G (IgG). These receptors are involved in immune complex recognition and the regulation of cellular immune responses.

This antibody is intended for research applications involving mouse immune cell populations and Fc receptor detection. It may also be used in flow cytometry workflows requiring Fc receptor blocking or staining strategies, subject to the manufacturer's validated protocol.

Key Product Features

• Target: Mouse CD16 and CD32.
• Fluorescent label: FITC (fluorescein isothiocyanate).
• Antibody type: Monoclonal antibody.
• Host species: Rat.
• Reactivity: Mouse.
• Application: Flow cytometry (FC).
• Concentration: 0.5 mg/mL.
• Pack size: 25 µg.
• Storage: 2–8 °C, protected from prolonged light exposure.
• Intended use: Research use only.

Biological Background: CD16 and CD32

CD16 and CD32 belong to the Fc gamma receptor family, which recognizes the Fc region of IgG antibodies. These receptors help immune cells detect antibody-bound targets and regulate downstream immune responses.

CD16 is a low-affinity IgG Fc receptor III, while CD32 is an IgG Fc receptor II. Their expression and function vary among immune cell types and receptor subtypes.

According to the supplied product information, CD16/CD32 expression is associated with B cells, monocytes and macrophages, natural killer (NK) cells, granulocytes, mast cells, and dendritic cells. Expression patterns depend on the specific receptor, cell type, and biological context.

Role of Fc Gamma Receptors in Immunology

Technical Specifications

Research Applications

• Flow cytometric detection of mouse CD16/CD32-associated Fc receptor expression.
• Immunophenotyping of mouse immune cell populations.
• Research into Fc gamma receptor biology and immune complex recognition.
• Studies of immune cell signaling and antibody-mediated immune responses.
• Investigation of receptor-associated staining and Fc receptor blocking strategies, subject to protocol validation.

Storage and Handling

• Store at 2–8 °C.
• Protect the antibody from prolonged exposure to light to help preserve FITC fluorescence.
• Do not freeze the product.
• Follow the manufacturer's handling instructions and recommended storage period.
• Use appropriate laboratory protective equipment and procedures when handling the reagent.

Related Products

Other CD16/32 antibody formats may be available for different fluorescent channels or detection strategies. Check the corresponding product documentation to confirm the exact conjugate, pack size, and suitability for your experiment.